Coding

Part:BBa_K193600:Design

Designed by: Kazuaki Amikura   Group: iGEM09_Tokyo_Tech   (2009-10-14)


melA gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1170
    Illegal BsaI.rc site found at 475


Design Notes

We added restriction enzyme sites (Prefix-Suffix) to the upstream and down stream of the ORF of melA.


Source

The gene sequence was obtained from GENEART.

References